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KMID : 0895820200300040031
Journal of Oriental Rehabilitation Medicine
2020 Volume.30 No. 4 p.31 ~ p.39
Comparison of Ingredients and Activities of Danggwisoo-san and Jakyakgamcho-tang by Extraction Method
Lee Dae-Yeon

Lee Ho-Sung
Jo Ju-Hwi
Yi Young-Woo
Kim Sung-Jin
Kang Kyung-Rae
Kwon Tae-Wook
Yang Seung-Gu
Lee In-Hee
Abstract
Objectives: Danggwisoo-san and Jakyakgamcho-tang are frequently prescribed for traffic accident patients in Korea. The aim of this study was to examine index compound analysis, antioxidant activity and amount of starch measurement by extraction method.

Methods: Danggwisoo-san and Jakyakgamcho-tang were extracted with water and 70% ethanol. Antioxidant activity was measured by 2,2-Diphenyl-1-picrylhydrazyl, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and ferric reducing antioxidant power according to the standard protocol. The contents of the indicator components nodakenin, paeoniflorin, and glycyrrhizin were measured by high-performance liquid chromatography, respectively. All starches were hydrolyzed and then total D-glucose was measured and compared.

Results: Antioxidant activity was excellent in 70% ethanol in all assays. The index component was jagged because its solubility was different depending on the extraction solvent. Starch content was significantly lower in 70% alcohol extract than water extract.
Conclusions The results of this study showed that physiological activities and components are different according to
extraction: conditions. Each herbal medicine has a suitable extraction solvent. Also, the difference in starch content is an object to be considered as it may affect digestion and absorption.
KEYWORD
Traffic accidents, Starch, Antioxidants, High pressure liquid chromatography
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